医用钛板表面涂层2-MPC对细菌生物膜形成的影响

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Abstract Objective To explore the effect of medical titanium plate surfacial coating 2-MPC on the formation of bacterial biofilm.Methods The surface of titanium plates were coated with 2-MPC using chemical grafting method and its morphology was observed by scanning electron microscopy.With standard strains of Staphylococcus aureus (ATCC25923) as an experimental bacteria，the titanium plates surfacial coating 2-MPC(experimental group) and normal plates(control group) were co-cultured into 5mL bacterial liquid with 1.5×10⁶cfu/mL bacteria concentration.When cultured for 24h and 48h，the viable bacteria adhered on the surface experimental group and control group were counted and scanned by electron microscopy.Results Scanning electron microscopy results showed that titanium plate surface coated by 2-MPC which prepared by chemical grafting method was smooth，with its original texture disappear.In vitro when cultured for 24h and 48h，the quantity of viable bacteria in the experimental group was significantly less than that in the control group(P＜0.05).Scanning electron microscopy observations showed that at 24h and 48h，the amounts of adhesional bacterial and formatted biofilm in experimental group were significantly less than that in the control group.(P＜0.05).And the numbers of formatted bacterial biofilm in the experimental group were significantly less than that in the control group at 48h.Conclusion The chemical grafting method can make 2-MPC coated on titanium plate surface effectively，and 2-MPC coating can effectively inhibit bacterial adhesion to titanium plate surface，and prevent the bacterial biofilm’s formation on the titanium plate surface.

Key words： titanium plate staphylococcus aureus bacterial biofilm infection 2-methacryloyloxyethyl phosphorylcholine

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