miR-31-5p靶向Notch1调节骨关节炎软骨细胞增殖和凋亡的作用

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Abstract Objective To identify the effects of miR-31-5p on osteoarthritis chondrocytes.Methods Osteoarthritis models of rats were established according to Hulth method，and primary chondrocytes were isolated and treated by interleukin 1β (IL-1β).The expression of miR-31-5p was evaluated by qRT-PCR.The potential target of miR-31-5p was predicted by TargetScanHuman database，and verified by luciferase reporter assay.Effects of miR-31-5p on proliferation and apoptosis of primary chondrocytes were determined by Cell Counting Kit-8 (CCK-8) and Flow Cytometry.Results The expression of miR-31-5p was downregulated in osteoarthritis models of rats.miR-31-5p mimics promoted proliferation of chondrocytes，and miR-31-5p inhibitor led to apoptosis of chondrocytes.Moreover，miR-31-5p inhibitor promoted expression of IL-6 and matrix metalloprotein 13 (MMP-13).Further investigation revealed Notch1 was the direct target of miR-31-5p，and Notch1 overexpression partially limited the protective role of miR-31-5p mimics in chondrocytes.Conclusion The downregulated miR-31-5p mediated apoptosis and limited proliferation of chondrocytes in osteoarthritis，which might contribute to pathogenesis of osteoarthritis.

Key words： osteoarthritis chondrocytes miR-31-5p Notch1

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	Articles by authors
	Wen Lianghua 1
	Chen Shihua 1
	Shou Zhexing 2

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Wen Lianghua 1,Chen Shihua 1,Shou Zhexing 2. Effects of miR-31-5p on Proliferation and Apoptosis of Osteoarthritis Chondrocytes by Targeting Notch1[J]. sygkzz, 2020, 26(5): 421-427.

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http://www.sygkzz.com/EN/ OR http://www.sygkzz.com/EN/Y2020/V26/I5/421

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