Abstract:Objective To investigate the effects of extracorporeal fucosylation of CD44 on the homing ability of rabbit bone marrow mesenchymal stem cells.Methods First,BMSCs were constructed to stably express enhanced green fluorescent protein (EGFP).Then,BMSCs were subjected to fucosylation treatment in vitro.Finally,BMSCs were injected intravenously into the model rabbits with tibial fractures.After 6 weeks of injection,the levels of stromal cell-derived factor (SDF-1) and monocyte chemoattractant protein-1 (MCP-1) in rabbit serum were detected by ELISA.The expression levels of SDF-1 and MCP-1 protein in damaged bone tissue were detected by Western Blot.The positive rate of EGFP expression was detected by staining.Results The results of fluorescence microscopy showed that BMSCs with stable expression of EGFP were successfully constructed.The results of ELISA and Western Blot showed that the secretion of SDF-1 and MCP-1 and the expression of SDF-1 and MCP-1 protein in BMSCs treatment group processed by fucosylated were significantly higher than those in BMSCs treatment group processed byno fucosylated (P<0.01).The results of immunohistochemical staining showed that the positive rate of EGFP expression was also significantly increased (P<0.05),which indicated that the BMSCs at the injured bone tissue were significantly increased and helpful in the repair of bone injury.Conclusion Extracorporeal fucosylationof CD44 molecules can significantly enhance the homing ability of rabbit BMSCs,which may be achieved by SDF-1 and MCP-1 regulation.
[1]Derubeis AR,Cancedda R.Bone marrow stromal cells (BMSCs) in bone engineering:limitations and recent advances[J].Ann Biomed Eng,2004,32(1):160-165.
[2]Wang H,He XQ,Jin T,et al.Wnt 11 plays an important role in the osteogenesis of human mesenchymal stem cells in a PHA/FN/ALG composite scaffold:possible treatment for infected bone defect[J].Stem Cell Res Ther,2016,7(1):1-13.
[3]彭敏锋,李剑.骨髓间充质干细胞与组织损伤修复的研究进展[J].中国组织工程研究,2008,12(8):1517-1520.
[4]Zhang LX,Shen LL,Ge SH,et al.Systemic BMSC homing in the regeneration of pulp-like tissue and the enhancing effect of stromal cell-derived factor-1 on BMSC homing[J].Int J Clin Exp Pathol,2015,8(9):10261-10271.
[5]Krause DS,Lazarides K,Van Etten RA,et al.Requirement for CD44 in homing and engraftment of BCR-ABL-expressing leukemic stem cells[J].Nat Med,2006,12(10):1175-1180.
[6]Dimitroff CJ,Lee JY,Rafii S,et al.CD44 is a major E-selectin ligand on human hematopoietic progenitor cells[J].J Cell Biol,2001,153(6):1277-1286.
[7]谢文鹏,毕荣修.骨髓间充质干细胞治疗骨折的临床研究进展[J].中国民族民间医药,2015,24(16):32-32.
[8]Ponte AL,Marais E,Gallay N,et al.The in vitro migration capacity of human bone marrow mesenchymal stem cells:comparison of chemokine and growth factor chemotactic activities[J].Stem Cells,2007,25(7):1737-1745.
[9]Huang H,Zhang X,Hu X,et al.A functional biphasic biomaterial homing mesenchymal stem cells for in vivo cartilage regeneration[J].Biomaterials,2014,35(36):9608-9619.
[10]Sackstein R,Merzaban JS,Cain DW,et al.Ex vivo glycan engineering of CD44 programs human multipotent mesenchymal stromal cell trafficking to bone[J].Nat Med,2008,14(2):181-187.
[11]Bolno PB,Morgan D,Wechsler A,et al.Chemokine induced migration of human mesenchymal stem cells:A strategy for directing cardiac repair[J].J Am Coll,2004,199(3):33.
[12]Zhou B,Han ZC,Poon MC,et al.Mesenchymal stem/stromal cells (MSC) transfected with stromal derived factor 1 (SDF-1) for therapeutic neovascularization:enhancement of cell recruitment and entrapment[J].Med Hypotheses,2007,68(6):1268-1271.
[13]刘举,周红雨,刘立斌,等.单核细胞趋化蛋白1体外趋化经成肌诱导分化后的小鼠BMSCs实验研究[J].中国修复重建外科杂志,2010,24(7):834-837.
[14]Sarkar D,Vemula PK,Zhao W,et al.Engineered mesenchymal stem cells with self-assembled vesicles for systemic cell targeting[J].Biomaterials,2010,31(19):5266-5274.
[15]Sackstein R.Glycoengineering of HCELL,the human bone marrow homing receptor:sweetly programming cell migration[J].Ann Biomed Eng,2012,40(4):766-776.