Objective  To study the mechanism of tibial transverse transport technology enhancing the regeneration，healing and repair of severe diabetic foot ulcers though activation of local hair follicle stem cell.Methods  We selected 126 severely infected diabetic foot patients who underwent tibial transverse transport in the Department of Osteopathic and Joint Surgery of the First Affiliated Hospital of Guangxi Medical University from June 2014 to January 2018.According to Wagner classification，there were 67 cases of grade Ⅲ，57 cases of grade Ⅳ and 2 cases of grade V.According to TEXAS classification structure，there were 50 cases of grade 2B，6 cases of grade 2C，9 cases of grade 2D，17 cases of grade 3B，1 case of grade 3C，and 43 cases of grade 3D.The features of wound healing was assessed.The edge tissues of the wound were taken before and after treatment，and HE staining was performed to observe the histological changes of the wound.Immunohistochemical staining was used to detect the expression and distribution of the Leucine-rich repeat-containing G protein-coupled receptor 5（LGR5） and cytokeratin 19 (CK19) protein.Results  The average healing time of 126 patients with severe diabetic foot was (143.5±86.4) days.The wound edge contracted early after removal，where surface was covered with yellow fibrinoid inflammation exudation; red granulation tissue gradually appeared after dressing change，while the peripheral epithelial tissue proliferated and shifted to the center.The wound was covered and linear or striped scars were remained.The complete layered structure of the epidermis，a large number of microvessels，collagen fibers and glandular structures were seen in edge tissues of the wound.Compared with the tissues of preoperative，the necrotic tissue and the inflammatory cell infiltration in the dermis were reduced.After treatment，the ratio of LGR5 to CK19 positive cell area were (6.781±1.889)% and (9.095±2.926)%，respectively，which were significantly higher than the positive area ratio of LGR5 (1.398±0.620)% and CK19 (1.834±0.772)% in the preoperative wound tissue(P＜0.05).Conclusion  Tibia transverse transport can promote the regeneration and healing of diabetic foot ulcer wounds.The mechanism may associated with regulating skin stem cells to participate in the wound process through activating positive cells such as LGR5 and CK19.