医用钛板表面涂层2-MPC对细菌生物膜形成的影响

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摘要目的探讨医用钛板表面涂层2-甲基丙烯酰氧基乙基磷酰胆碱(2-methacryloyloxyethyl phosphorylcholine，2-MPC)对金黄色葡萄球菌生物膜形成的影响。方法利用化学接枝法制备钛板表面2-MPC涂层并用扫描电镜观察其表面形貌，以金黄色葡萄球菌(ATCC25923)标准菌株作为实验用菌，将已消毒的2-MPC涂层钛板(实验组)及普通钛板(对照组)置入5 mL细菌浓度为1.5×10⁶cfu/mL的菌液中共同培养。分别于体外培养后24 h、48 h行膜内活菌计数和扫描电镜观察。结果扫描电镜观察显示通过化学接枝法制备的2-MPC涂层钛板表面形貌光滑，钛板原先的纹理消失。体外培养24 h、48 h实验组钛板表面活菌计数数量均显著少于对照组(P＜0.05)；扫描电镜观察发现24 h、48 h实验组钛板表面细菌黏附量均明显少于对照组(P＜0.05)，且在48 h时实验组钛板表面菌膜形成量明显少于对照组。结论化学接枝法能有效将2-MPC涂层于钛板表面，且2-MPC涂层可以有效抑制钛板表面细菌黏附，预防钛板表面细菌生物膜的形成。

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	李舟 1
	杨明坤 1
	何坤林 1
	王靖 1
	刘泯邑 1
	柯明池 2
	王业华 2

关键词 ：钛板, 金黄色葡萄球菌, 生物膜, 感染, 2-甲基丙烯酰氧基乙基磷酰胆碱

Abstract：Objective To explore the effect of medical titanium plate surfacial coating 2-MPC on the formation of bacterial biofilm.Methods The surface of titanium plates were coated with 2-MPC using chemical grafting method and its morphology was observed by scanning electron microscopy.With standard strains of Staphylococcus aureus (ATCC25923) as an experimental bacteria，the titanium plates surfacial coating 2-MPC(experimental group) and normal plates(control group) were co-cultured into 5mL bacterial liquid with 1.5×10⁶cfu/mL bacteria concentration.When cultured for 24h and 48h，the viable bacteria adhered on the surface experimental group and control group were counted and scanned by electron microscopy.Results Scanning electron microscopy results showed that titanium plate surface coated by 2-MPC which prepared by chemical grafting method was smooth，with its original texture disappear.In vitro when cultured for 24h and 48h，the quantity of viable bacteria in the experimental group was significantly less than that in the control group(P＜0.05).Scanning electron microscopy observations showed that at 24h and 48h，the amounts of adhesional bacterial and formatted biofilm in experimental group were significantly less than that in the control group.(P＜0.05).And the numbers of formatted bacterial biofilm in the experimental group were significantly less than that in the control group at 48h.Conclusion The chemical grafting method can make 2-MPC coated on titanium plate surface effectively，and 2-MPC coating can effectively inhibit bacterial adhesion to titanium plate surface，and prevent the bacterial biofilm’s formation on the titanium plate surface.

Key words： titanium plate staphylococcus aureus bacterial biofilm infection 2-methacryloyloxyethyl phosphorylcholine

作者简介: 李舟(1986－ )，男，主治医师，四川省巴州市通江新区医院骨科，636057。

引用本文:

李舟 1，杨明坤 1，何坤林 1，王靖 1，刘泯邑 1，柯明池 2，王业华 2. 医用钛板表面涂层2-MPC对细菌生物膜形成的影响[J]. 实用骨科杂志, 2020, 26(6): 513-516.

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http://www.sygkzz.com/CN/ 或 http://www.sygkzz.com/CN/Y2020/V26/I6/513

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